博士屯教育 | 名校科研:從機械到生物,我參加美國科研

來源:SOHU  [  作者:博士屯教育   ]  責編:楊麗  |  侵權/違法舉報

原標題:博士屯教育 | 名校科研:從機械到生物,我參加美國科研

秉持尊重學生和家長隱私的原則,以下所展示的信息,已經去掉敏感和涉及知識產權的信息。

還有N多學術素材,由于知識產權或家長原因,不便公開,敬請理解。

本文作者M同學,來自北京理工大學,機械工程專業,大學二年級,成績優異,計劃本科畢業后赴美讀研究生,考慮到以后的就業情況,希望攻讀生物醫學工程相關專業研究生,轉專業申請研究生,相比于本專業而言有一些困難,為了獲得名校的錄取,增加申請競爭力,為此報名了美國名校科研生物醫學工程專業,參與選拔。參加美國名校科研,增加學術背景,開拓視野,獲得真知。

選擇在大二結束后的暑假參與科研,有獨特的優勢,學生表現優異,就成為了教授課題組的一員,以后大三、大四可以繼續深入進行科研,這樣會獲得連續性的科研履歷,更有利于美國頂尖大學研究生院的錄取。

M同學非常期待此次的科研,這是他第一次來到世界頂尖名校的實驗室里做科研。既期待又緊張,因為是跨專業,M同學私下閱讀了很多生物醫學工程相關的書籍,加強專業履歷和技能背景,增加未來名校錄取的競爭力。我們非常高興地看到,學生在科研感受中寫道:“我非常享受美國科研的過程,過的非常充實并且學到了很多專業相關的最前沿的知識和研究技能,也發現了很多自己需要提升的地方,更加堅信了自己未來的專業選擇和人生道路。”

作為科研申請老師,我們想告訴每位參與美國科研的孩子,獲得推薦信、科研證書、科研報告、科研履歷,其實僅僅是美國科研的基礎性收獲。更為重要的是,學生通過與頂尖學者、專家的交流,樹立了長期的學術目標,讓學術能力伴隨著生命的成長而發展,讓學術贊美生命!

美國科研就是背景提升,目標就是提高本,碩,博錄取質量。

科研總結

本文是學生在美國大學科研學習結束后所寫的感受,并在文后附錄了學生的推薦信,供學生及家長參考。

注:中文譯文是編輯進行的整理,英語能力較好的學生和家長建議直接閱讀英文。

第一周

This week is the first I am working on a research project at American University. In the first week, I learned many basic skills in the lab. I learned how to use the pipette correctly. I thought it was very easy at first because I’ve used in my senior high school, but when I use it I found I was wrong. It needs to be accurate and it is very difficult especially use the pipette to have 1ul solution. I even got 0.5ul by mistakes. By trying it for lots of times, I can use it much well. Then I learn to mix a buffer A for HPLC. I know the composition of it and calculate the mass of each one the I dissolve each and use the PH detector to adjust the PH of it to 7. It’s the first time I have to make a reagent and it helps me know we need to think thoroughly about how much of each component should we add.

這周是我在美國大學進行的第一個研究項目。第一周,我在實驗室學到了很多基本技能,我學會了如何正確使用吸液管。一開始我覺得這很容易,因為我在高中就用過,但當我用的時候,我發現我錯了。它需要準確,特別是使用移液管獲得1ul溶液非常困難。我甚至因為錯誤而得到了0.5ul。通過多次嘗試,我可以很好地使用它。然后我學習混合一種緩沖液A用于高效液相色譜。我知道它的成分,并計算出每一種物質的質量,我溶解每一種物質,然后用酸堿度檢測器把它的酸堿度調整到7。這是我第一次做試劑,這有助于我知道我們需要仔細考慮每種成分應該添加多少。

Then came the second day. I make the standard mixture for the HPLC. As I got the instruction, I think it’s a quite difficult one. First, I need to dissolve all the compounds into 2ml stock solution. I need to consider the purity of each compound. It really needs my patience because I need to fetch only 2mg and it needs to be very accurate. After I finished it, I need to calculate the volume needed for each one to make the mixture. I used my pen and calculate by hand at first, but my professor taught me to use excel to do the calculation, I soon learned how to do it and found it much more convenient. In the end, I adjust pH around 7 with pH paper, and that’s an easy task.

第二天來了。我為HPLC制作標準混合物。當我得到指示時,我認為這是一個非常困難的指令。首先,我需要將所有化合物溶解到2ml儲備溶液中。我需要考慮每種化合物的純度。它真的需要我的耐心,因為我只需要獲取2毫克,它需要非常準確。完成之后,我需要計算每個混合物所需的體積。我一開始用筆和手工計算,但是我的教授教我使用excel進行計算,我很快就學會了如何去做,發現它更方便。最后,我用pH試紙調節pH值約為7,這是一項簡單的任務。

Before the third day, I used Google to search how does the HPLC work. And I know the function of each part of HPLC. One that day, I try to operate the HPLC. It was a little nervous because I always heard people saying that HPLC is difficult. However, when my professor gave me the guidance of it and show me how to do that, it became much easier. I follow the guidance and prime the pumps and then purge them using methanol, water and then buffers. Then I run the However standard mixture I made yesterday for the HPLC to analyze it. I firstly used the 50ul standard mixture. But after I had my lunch, there is something wrong with the computer and the data loss. I had to do it again and wait for 2 hours again. That’s frustrating. Finally, I successfully got the result below. Then I try 100ul as well then go the same result. It means that my standard mixture is the correct one.

在第三天之前,我用谷歌搜索高效液相色譜是如何工作的。我知道高效液相色譜各部分的功能。一天,我試著操作高效液相色譜法。有點緊張,因為我經常聽到人們說高效液相色譜法很難。然而,當我的教授給我指導并告訴我如何做的時候,事情變得簡單多了。我遵循指導,啟動泵,然后用甲醇、水和緩沖器進行清洗。然后我運行昨天為高效液相色譜分析而制備的標準混合物。我第一次使用50ul標準混合物。但是在我吃過午飯后,電腦出了點問題,數據丟失了。我得再做一次,再等兩個小時。真令人沮喪。最后,我成功地得到了下面的結果。然后我也嘗試100ul,然后得到相同的結果。這意味著我的標準混合物是正確的。

I feel really excited about it’s the first time that I operate HPLC. Through the first week, I find it’s very interesting to do a project in the lab. I can learn much more things than in traditional classes. And I also know I should be more patient. What’s more, the most important thing I learned from the first week is the powerful function of excel. And I will learn to use excel more fluently in my daily study.

我感到非常興奮,因為這是我第一次操作高效液相色譜。在第一個星期里,我發現在實驗室里做一個項目很有趣。比起傳統的課程,我能學到更多的東西。我也知道我應該更有耐心。而且,我從第一周學到的最重要的是Excel的強大功能。我將學會在日常學習中更流利地使用Excel。

Week 2

This week is the second week of doing research at American University.This week I continued my HPLC job. Things have been much more similar to me than the first week. Everything turns great this week. On the first day, I finish my first sample and protein. I followed the instruction provided by my professor. I firstly made some preparation such as having the tubes ready and preparing other compounds. Then I completed the sample1 step by step with the help of my professor. It’s the first time for me to see liquid nitrogen, and that’s really fantastic. Meanwhile, I also did the 20ul and 100ul injection of my standard mixture. Then on the second day, I continued my HPLC project. I injected 40ul and 60ul standard mixture. After several days’ practice, I can operate the machine much faster. At the same time, I made two samples of the tissue. It’s the major task I could do in this project. It’s quite difficult for me because I need to use the liquid nitrogen and I need to be really patient for it has some danger. Finally, I made it following the instruction. I am really delighted as I finish the task.

這周是在美國大學做研究的第二周,這周我繼續我的高效液相色譜工作。事情比第一周更像我。這周一切都變好了。第一天,我完成了我的第一個樣品和蛋白質。我聽從教授的指示。我首先做了一些準備工作,比如準備好管子和準備其他化合物。然后,在我的教授的幫助下,我一步一步地完成了這個樣本。這是我第一次看到液氮,真是太棒了。同時,我還注射了20ul和100ul的標準混合物。第二天,我繼續我的高效液相色譜項目。我注射了40ul和60ul的標準混合物。經過幾天的練習,我能更快地操作機器。同時,我做了兩個組織樣本。這是我在這個項目中可以做的主要任務。這對我來說相當困難,因為我需要使用液氮,我需要非常耐心,因為它有一些危險。最后,我按照指示做了它。我很高興完成這項任務。

As the last day for this week came, I carried on my job for making samples 4,5,6,7 of the two groups. Meanwhile, I do my injections one by one. It becomes much easier to finish my job than I firstly finish the task. All the result seem great. In the end, I finished my job faster than we have thought. Therefore, the professor taught me how to analyse the data of the HPLC.

當這周的最后一天到來時,我繼續我的工作,在兩組中制作4,5,6,7個樣品。同時我一個接一個地注射。完成工作比我先完成任務容易得多。所有的結果都很好。最后,我比我們想象的更快地完成了我的工作。因此,教授教我如何分析高效液相色譜數據。

In this week the operation in the experiments became much and much easier for me. Because I know each step of the experiments. For example, when I finish the samples, all the steps are same for each sample. As I have finished more and more samples, I can behave much better and much faster. But I also know that speed is not what we need in the end, we need the accuracy. One standard mixture’s result seems strange after the injection. I think I may make some mistakes in the process. From this I realize that we should spend more time considering about how to make the correct result instead of how to do the experiments faster. I will mind that in my study in the future.

在這周,實驗的操作對我來說變得越來越簡單。因為我知道實驗的每一步。例如,當我完成樣本時,每個樣本的所有步驟都是相同的。因為我已經完成了越來越多的樣品,我可以表現得更好更快。但我也知道速度不是我們最終需要的,我們需要的是準確度。注射后一種標準混合物的結果似乎很奇怪。我想在這個過程中我可能會犯一些錯誤。由此我認識到,我們應該花更多的時間來考慮如何得到正確的結果,而不是如何更快地進行實驗。我以后在學習時會介意的。

Week3:

This week is the third week I have been studying at American University. This week, in general, I finish preparing the samples, continue injecting the samples and starting the Lowry Protein assay. Although something was not so satisfactory, I finally finish all the tasks. The first day is another day for making and injecting samples. I tried to operate the machine without the instruction to check whether I have mastered the steps correctly. At first, it bothers me a lot, I need to recall what I did last time and double-check it to ensure I don’t make a mistake. Gradually it turns much easier for me as I carry on my experiments. And everything shows great in the end. I make another 4 samples and inject 3 of them. Meanwhile, I also make some data analyses in my spare time. I think I master the skills well after two-weeks practice.

這周是我在美國大學學習的第三周。一般來說,這周我完成了樣品的準備,繼續注射樣品,開始了低蛋白分析。雖然有些事情不太令人滿意,但我最終完成了所有的任務,第一天又是制作和注射樣本的另一天。我試著在沒有指示的情況下操作機器,以檢查我是否正確掌握了這些步驟。一開始我很煩惱,我需要回憶我上次做的事情,然后再檢查一遍,以確保我不會犯錯誤。隨著實驗的進行,它逐漸變得對我來說容易多了。最后一切都很好。我又做了4個樣品,注射了3個。同時,我也在業余時間做了一些數據分析。我想經過兩周的練習,我掌握得很好。

On the second day I make the last three samples and finally finish my task for finishing 14 samples. I’m really proud of that because it’s my first time to do things like that. I find a lot of interests during I make those. Then I continue my injections as usual. Meanwhile, I finish my data analyses of the standard mixture. However, there is something wrong with that. Most of my standard mixtures only show 8 peaks instead of 9. It really confused me a lot. I dissolved 9 compounds in my standard mixture but why only show 8 peaks. Then my professor helps me with that. We check the nine compounds I made in the first day. And we find that the reason is that my Adenosine probably doesn’t dissolve in the resolution, that’s the missing peak. I think I might use the pure NH4OH to dissolve it. Then professor gives me his Adenosine data for me to calculate. I will put this in my mind. And I finally know the reason professor asked me to keep the each solution of every compounds.

第二天,我做了最后三個樣本,最后完成了14個樣本的任務。我真的為此感到驕傲,因為這是我第一次這樣做。我在制作過程中發現了很多興趣。然后像往常一樣繼續注射。同時,完成了標準混合物的數據分析。但是,這有點問題。我的大多數標準混合物只顯示8個峰值而不是9個。我真的很困惑。我在標準混合物中溶解了9種化合物,但為什么只有8個峰。然后我的教授幫我解決這個問題。我們檢查了我第一天制造的九種化合物。我們發現原因是我的腺苷可能不會溶解在分辨率中,這就是缺失的峰值。我想我可以用純NH4OH來溶解它。然后教授給我他的腺苷數據讓我計算。我會記住這一點。我終于知道教授讓我保留每種化合物的每一種溶液的原因。

Week 4

This week is the third week I have been studying at American University. And I must say time passed so quickly and I really enjoy this experience because I think I like using my hands to do some experiments. And this week I finished all my sample injections and analyzed all the data and make the tables of them.

本周是我在美國大學學習的第三周。而且我必須說時間過得如此之快,我真的很喜歡這種體驗,因為我覺得我喜歡用手來做一些實驗。本周我完成了所有樣品注射并分析了所有數據并制作了表格。

On the first day, A good news for today is that the HPLC’s problem has been solved, so I carry on my injections. And I have finished the last four samples and successfully get the result. Then comes the data analyses. Professor introduces a new software called Graphpad prism to me. That is really powerful and can produce the curves according to the data. I soon learn to operate it. I’m really glad to master another useful tool for analyzing the data.

在第一天,今天的好消息是HPLC的問題已經解決,所以我繼續我的注射。我已完成最后四個樣本并成功獲得結果。然后是數據分析。教授向我介紹了一款名為Graphpad prism的新軟件。這非常強大,可以根據數據生成曲線。我很快學會了操作它。我很高興掌握另一種分析數據的有用工具。

Then came the second day and sadly my last day for experiments. Professor He then introduced the method to figure out each peak stands for which composition. I soon got it and started to analyse all the samples. After one-morning’s effort, I finally got all the data in an excel. And professor showed me how to use Graphpad prism to deal with the data. And then I got the graphs of them. However, something wrong happened. Some of my data failed to show the correct trend and much larger than others. As I looked back on my experiment, I thought there was something wrong with my protein assay. It was really frustrated and disappointed. But professor He told me on the first day that error is not a bad thing and you should learn from the error. I think this is very meaningful and I think I should be more patient to do anything so that I could make less mistakes.

第二天來了,可悲的是我最后一天的實驗。何教授然后介紹了找出每個峰值代表哪種成分的方法。我很快得到它并開始分析所有樣品。經過一個上午的努力,我終于獲得了excel中的所有數據。教授向我展示了如何使用Graphpad棱鏡來處理數據。然后我得到了他們的圖表。但是,發生了一些錯誤。我的一些數據未能顯示正確的趨勢,并且比其他數據大得多。當我回顧我的實驗時,我認為我的蛋白質分析有問題。真的很沮喪和失望。但是教授在第一天就告訴我錯誤不是壞事,你應該從錯誤中吸取教訓。我認為這是非常有意義的,我認為我應該更耐心地做任何事情,以便減少錯誤。

Then my internship ends. I think I do gained a lot from this experience. I know the basic process and methods of research in the US and I realize how precise is required in any research, which need to be really patient. Also, I have made a lot of friends during this experience and know a lot about city. What’s most important to me is that I have know more about Medical area and I can have more choices when I decide my major in the future after graduation.

I really enjoy this experience. I’m very grateful for professor He to give me a chance to have this experience and give me very patient instruction.

然后我的實習結束了。我想我從這次經歷中獲得了很多。我知道美國的基本過程和研究方法,并且我意識到在任何需要真正耐心的研究中需要多么精確。此外,我在這次經歷中結識了很多朋友,并且對城市了解很多。對我來說最重要的是,我對醫學領域有了更多的了解,畢業后我決定將來的專業可以有更多的選擇。

我真的很喜歡這種體驗。我非常感謝教授讓我有機會獲得這種經驗并給予我非常耐心的指導

科研報告 (節選)

科研學習中,在美國教授的輔導下,M學生完成的專業科研報告(節選)

推薦信(節選)

First, I have been repeatedly impressed by his outstanding research ability. He was both driven and teachable, willing to invest considerable time and energy into his work and open to receive instructions to refine his skills. He is a quick learner, able to readily assimilate the goals and methods of the tasks he was assigned to. For example, within a short period, he mastered the correct usage of the pipette, learned to prepare a solution named buffer A for HPLC, familiarized himself with the functions of HPLC, and acquired the skills of processing data with Excel and Graphpad prism. Besides, he applied himself diligently and with enthusiasm throughout the whole research program by flexibly practicing what he had gained in the first stage of our research. I believe that he has made significant progress at American University, and the final report with logical structure and vivid graphs was a strong proof of his harvest.

首先,我對他杰出的研究能力印象深刻。他既有動力,又有教益,愿意在工作中投入大量的時間和精力,愿意接受指導來提高他的技能。他學得很快,能夠很容易地吸收他所分配任務的目標和方法。例如,在很短的時間內,他掌握了移液管的正確使用方法,學會了制備高效液相色譜用緩沖液A,熟悉了高效液相色譜的功能,掌握了用Excel和graphpad prism處理數據的技巧。此外,他在整個研究項目中勤奮而熱情地運用自己,靈活地實踐他在我們研究的第一階段所獲得的成果。我相信他在美國大學科研期間取得了顯著的進步,最終的報告用邏輯結構和生動的圖表證明了他的收獲。

Second, I appreciate his positive attitude towards research. It has been clear to me that he enjoys research a great deal. He often used the word “interesting” to describe his experiment, and considered it an awesome experience when firstly applied liquid nitrogen in his experiment. When encountered with problems during the research process, he never complained; instead, he positively attempted to get through it by himself first and provided multiple solutions. Later, he would discuss with me and revise his solutions accordingly. He thought mistakes were not bad, while lessons drawn from mistakes were most valuable. I think such a proactive research attitude is the cornerstone for him to be an excellent researcher in the near future.

第二,我欣賞他對研究的積極態度。我很清楚他很喜歡研究。他經常用“有趣”這個詞來描述他的實驗,并認為這是一個了不起的經驗時,首次應用液氮在他的實驗。在研究過程中遇到問題時,他從不抱怨;相反,他積極嘗試自己先解決問題,并提供了多種解決方案。稍后,他會和我討論并相應地修改他的解決方案。他認為錯誤并不壞,而從錯誤中吸取的教訓是最有價值的。我認為這種積極主動的研究態度是他在不久的將來成為一名優秀研究員的。

選拔流程

1.提交報名表

2.科研組擇優面試

3.面試通過后,發送錄取確認書

4.協調機票、接機、住宿;

5.赴美開始科研

6.獲得導師推薦信,科研證書,豐富的CV、PS履歷

學生報名美國名校科研項目的當天,名校科研的后期申請老師會開始面試輔導,并會在未來7天內安排學生與美方大學科研組導師的視頻面試。

科研周期

每期時間長度為3—4周;

(針對假期只有2周的申請者,可申請2周實地+2周遠程,確保科研收獲)

具體情況根據學生面試情況由美方進行調整;

報名后協調安排面試,面試前輔導學生閱讀1篇專業論文;

美國名校科研,讓學術贊美生命!

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博士屯教育,專注美國名校合作,為學生定制有利于成長的一站式背景提升方案,助力本碩博名校錄取。

博士屯,就是做背景提升的,目標就是提高本、碩、博錄取質量。 想

要了解名校科研詳情,可以+名校科研-科研顧問-vx:330809108,進行咨詢。

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